Methods in Biotechnology 4 - Natural Products Isolation (Humana Press, 1998).pdf

Researchers from both industry and academia present in detail techniques for the extraction and isolation of natural products of all classes. These experienced experimentalists describe a wide array of methodologies, including basic chromatography, ion-exchange methods, HPLC, countercurrent and planar chromatography, SFE, and crystallization. They also address the specific problems involved in working with plants, marine organisms, water-soluble compounds, scale-up, dereplication and partial identification, and follow-up of a natural product lead. For the less-experienced they offer hands-on advice - using real examples - about how to approach an extraction in general, when and why to apply a particular technique, how to carry out the methods described, and how to modify conditions according to the compound being isolated.